Collink.3D™ Human Collagen BioInk
Collink.3D™ 50 is based on recombinant human type I collagen (rhCollagen), chemically modified to generate a curable bioink with approximately 50% of the primary amines converted into methacrylamides.
What are the uses of Collink.3D™?
Collink.3D™ enables fabrication of 3D constructs with complex architectures, meeting the demand for specific geometric, physical and biological properties. Biofabricated constructs made with Collink.3D™ can be used in a wide range of soft and hard tissue applications, including tissue models for drug discovery and screening, as well as biofabrication of tissues and organs
What are the applications of Collink.3D™ 50?
Collink.3D™ 50 enables fabrication of 3D constructs with complex architectures, meeting the need for specific geometric, physical and biological properties. Biofabricated constructs made with Collink.3D™ can be used in a wide range of soft and hard tissue applications, including tissue models for drug discovery and screening, as well as biofabrication of tissues and organs.
As a result of its cytocompatibility, Collink.3D™ 50 supports viability of various cell types, including fibroblasts, stem cells, induced pluripotent stem cells, endothelial and epithelial cells.
Degree of methacrylation corresponds to mechanical properties especially stiffness. Collink.3D™ 50 provides softer hydrogels while Collink.3D™ 90 supports stiffer constructs.
Collink.3D™ 50 specifications
Parameter | Specifications |
|---|---|
pH | 1.9 – 2.4 |
Appearance | Clear, transparent, no visible particles |
Identify | Type 1 Collagen
|
Purity | > 95% Type 1 Collagen
|
Concentration | 13 – 17 mg/ml |
Degree of Functionalization | 45 – 65% |
Total Aerobic Microbial Counts | < 10 CFU/ml |
Total Yeast and Mold Counts | < 10 CFU/ml |
Endotoxins | < 7 EU/ml |
Elemental analysis | According to USP<233> |
Curing Kinetics – Time to G’ >100 Pa | ≤ 5 sec |
Why Collink.3D™ 50?
Animal-free
Optimal rheology at room temperature
Supports high viability of different cell types
Biocompatible, non-immunogenic
Fluorescent micrographs of cells grown on Collink.3D™ 50 layer [A-C] and 3D-printed constructs [D-F]. CD31 endothelial membrane specific staining of cells following 6 days in culture [A]. Human endothelial cells following 7 days in culture [B]. Human fibroblasts following 1 day in culture [C] . Human fibroblasts proliferated over a 3D-printed disc [D] and mesh [E] following 10 days of incubation. Spheroid of human fibroblasts embedded in Collink.3D™ 50 following 7 days incubation [F]. Cells stained with DAPI (nuclei) and GFP (actin)
Collink.3D™ chemical, physical and
biological characterization
Extensive studies carried out to characterize the chemical, physical and
biological characteristics of Collink.3D™ confirmed its identity to human
type I collagen and its performance as a bioink.
Collink3D™: Ready, set, print!
For more information, contact us at Collink.3D@collplant.com