Collink.3D™ Human Collagen BioInk
Collink.3D™ 90 is based on recombinant human type I collagen (rhCollagen), chemically modified to generate a curable bioink with approximately 90% of the primary amines converted to methacrylamides. The high degree of functionalization results in stiffer gels, with increased mechanical properties.
What are the uses of Collink.3D™?
Collink.3D™ enables fabrication of 3D constructs with complex architectures, meeting the demand for specific geometric, physical and biological properties. Biofabricated constructs made with Collink.3D™ can be used in a wide range of soft and hard tissue applications, including tissue models for drug discovery and screening, as well as biofabrication of tissues and organs
What are the applications of Collink.3D™ 90?
Collink.3D™ 90 enables fabrication of 3D constructs with complex architectures, meeting the need for specific geometric, physical and biological properties. Biofabricated constructs made with Collink.3D™ 90 can be used in a wide range of soft and hard tissue applications, including tissue models for drug discovery and screening, as well as biofabrication of tissues and organs.
As a result of its cytocompatibility to its cytocompatibility, Collink.3D™ 90 supports viability of various cell types, including fibroblasts, stem cells, induced pluripotent stem cells, endothelial and epithelial cells.
Degree of methacrylation corresponds to mechanical properties especially stiffness. Collink.3D™ 50 provides softer hydrogels while Collink.3D™ 90 supports stiffer constructs.
Collink.3D™ 90 specifications
Parameter | Specifications |
|---|---|
pH | 1.9 – 2.4 |
Appearance | Clear, transparent, no visible particles |
Identify | Type 1 Collagen
|
Purity | > 95% Type 1 Collagen
|
Concentration | 13 – 17 mg/ml |
Degree of Functionalization | > 80% |
Total Aerobic Microbial Counts | < 10 CFU/ml |
Total Yeast and Mold Counts | < 10 CFU/ml |
Endotoxins | < 7 EU/ml |
Elemental analysis | According to USP<233> |
Curing Kinetics – Time to G’ >100 Pa | ≤ 2 sec |
Why Collink.3D™ 90?
Increased mechanical properties
Optimal rheology at room temperature
Supports high viability of different cell types
Biocompatible, non-immunogenic
Fluorescent micrographs of 3D cell culture of human fibroblasts within Collink.3D™ 90 hydrogel. Cells are fixated and stained with Hoechst and RFP. A. Fibroblasts invasion and migration into gel matrices five days after seeding: commercial standard. vs. B. Collink.3D™ 90. C.Fibroblasts embedded in Collink.3D™ 90 spheroid after a week of incubation at 37 °C.
Collink.3D™ chemical, physical and
biological characterization
Extensive studies carried out to characterize the chemical, physical and
biological characteristics of Collink.3D™ confirmed its identity to human
type I collagen and its performance as a bioink.
Collink3D™: Ready, set, print!
For more information, contact us at Collink.3D@collplant.com